Mechanical Disruption Methods Shearing
Vortexer Shearing with Beads: Though not their intended use, vortexers are routinely used to disrupt samples.This method relies on adding grinding beads and sample to a tube and then repeatedly vortexing. Typically used for the lysis of microorganisms, vortexers can also disrupt tissues by using large grinding beads ( 2 mm) made of zirconium or stainless steel.